董小鸥

教授，博士生导师

办公地址：澳门十大正规老牌网赌第三实验楼二期3层

电子邮箱：odong(at)njau.edu.cn

教育和工作经历

2006.9-2010.6   香港大学 本科（生物技术）

2010.6-2010.8   北京生命科学研究所(NIBS)  科研助理

2010.9-2016.8   加拿大英属哥伦比亚大学(UBC) 博士（植物学）

2016.9-2020.9   美国加州大学戴维斯分校(UC Davis) 博士后

2020.11-至今   澳门十大正规老牌网赌 教授

研究兴趣

基因编辑是生命科学领域的颠覆性技术，为生物育种提供了新的机遇。利用现存的基因编辑工具在作物基因组中的指定位点引入indel突变、大片段删除，或精准地替换单个或多个碱基的方法已相对成熟。然而，作物中大片段DNA定点插入或替换的操作往往面临效率低、片段长度不足、脱靶率高等挑战，制约了此类操作在育种中的广泛应用。针对这些问题，课题组致力于在作物中建立高效精准的大片段DNA定点插入技术，以满足更广泛的育种应用场景。

利用基因编辑技术可以在作物中创制难以通过自然突变或人工诱变等途径获得的遗传变异类型，并且变异位置明确、易于鉴定，为作物功能基因组学研究提供了便利。课题组利用高通量基因编辑技术，在水稻中挖掘具有育种利用价值的基因或变异，为绿色高效水稻新品种的培育提供原始创新。

现招聘博士后1名，开展作物基因编辑新方法研发，依托课题组申报澳门十大正规老牌网赌钟山青年研究员岗位，待遇从优。

执教课程

CROP4222W    作物育种中的基因定向插入与替换

CROP4201    作物育种学总论（作物基因工程及基因编辑部分）

CROP7201*     植物遗传改良理论与方法（植物基因工程部分，全英文）

发表论文

1. Huang J, Qiu ZY, He J, Xu HS, Wang K, Du HY, Gao D, Zhao WN, Sun QG, Wang YS, Wen PZ, Li Q, Dong XO, Xie XZ, Jiang L, Wang HY, Liu YQ, and Wan JM (2023). Phytochrome B mediates dim-light-reduced insect resistance by promoting the ethylene pathway in rice. Plant Physiology. 191(2):1272-1287.

2. Ercoli MF, Ramos PZ, Jain R, Pilotte J, Dong OX, Thompson T, Wells CI, Elkins JM, Edwards AM, Couñago RM, Drewry DH, and Ronald PC (2022). An open source plant kinase chemogenomics set. Plant Direct. 6(11):e460.

3. Dong OX and Ronald PC (2021). Targeted gene insertion in plants. PNAS.118(22):e2004834117.

4. Dong OX (2020). Gene Replacement and Knockin Made Easy in Plants. Molecular Plant. 13(8):1104.

5. McWhite CD, Papoulas O, Drew K, Cox RM, June V, Dong OX, Kwon T, Wan C, Salmi ML, Roux SJ, Browning KS, Chen ZJ, Ronald PC, and Marcotte EM (2020). A pan-plant protein complex map reveals deep conservation and novel assemblies. Cell. 181(2):460-474.

6. Dong OX, Shu Y, Jain R, Zhang N, Duong P, Butler C, Li Y, Lipzen A, Martin J, Barry J, Schmutz J, Tian L, and Ronald PC (2020). Marker-free carotenoid-enriched rice generated through targeted gene insertion using CRISPR-Cas9. Nature Communications. 11(1):1-10

7. Dong OX and Ronald PC (2020). Fast-track for engineered plants. Nature Biotechnology. 38(1):32-34.

8. Dong OX and Ronald PC (2019). Genetic Engineering for Disease Resistance in Plants: Recent Progress and Future Perspectives. Plant Physiology. 180(1):26-38.

9. Li Y, Cao XL, Zhu Y, Yang XM, Zhang KN, Xiao ZY, Wang H, Zhao JH, Zhang LL, Li GB, Zheng YP, Fan J, Wang J, Chen XQ, Wu XJ, Zhao JQ, Dong OX, Chen XW, Chern M, and Wang WM (2019). Osa-miR398b boosts H2O2 production and rice blast disease-resistance via multiple superoxide dismutases. New Phytologist. 222(3):1507-1522.

10. Wu Z, Li M, Dong OX, Xia S, Liang W, Bao Y, Wasteneys G, and Li X (2018). Differential regulation of TNL‐mediated immune signaling by redundant helper CNLs. New Phytologist. 222(2):938-953.

11. Dong OX#, Ao K#, Xu F#, Johnson K, Wu Y, Li L, Xia S, Liu Y, Huang Y, Xia S, Rodriguez E, Chen X, Chen S, Zhang Y, Petersen M, and Li X (2018). Individual components of paired typical NLR immune receptors are regulated by distinct E3 ligases. Nature Plants. 4(9):699-710.

12. Dong OX#, Tong M#, Bonardi V, Kasmi EK, Woloshen V, Wunsch LK, Dangl JL, and Li X (2016). TNL-mediated immunity in Arabidopsis requires complex regulation of the redundant ADR1 family. New Phytologist. 210(3):960-73.

13. Dong OX, Meteignier LV, Plourde MB, Ahmed B, Wang M, Jensen C, Jin H, Moffett P, Li X, and Germain H (2016). Arabidopsis TAF15b localizes to RNA processing bodies and contributes to snc1-mediated autoimmunity. Molecular Plant-Microbe Interactions. 29(4):247-57.

14. Huang S, Monaghan J, Zhong X, Lin L, Sun T, Dong OX, and Li X (2014). HSP90s are required for NLR immune receptor accumulation in Arabidopsis. The Plant Journal. 79(3):427-439.

15. Johnson K, Dong OX, Huang Y, and Li X (2013). A Rolling Stone Gathers No Moss, but Resistant Plants Must Gather Their MOSes. Cold Spring Harbour Symposia on Quantitative Biology. 77: 259-68.

16. Liu J, Ding P, Sun T, Nitta Y, Dong OX, Huang X, Yang W, Li X, Botella JR, and Zhang Y (2013). Heterotrimeric g proteins serve as a converging point in plant defense signaling activated by multiple receptor-like kinases. Plant Physiology. 161(4): 2146-58.

17. Kong Q, Qu N, Gao M, Zhang Z, Ding X, Yang F, Li Y, Dong OX, Chen S, Li X, and Zhang Y (2012). The MEKK1-MKK1/MKK2-MPK4 kinase cascade negatively regulates immunity mediated by a mitogen-activated protein kinase kinase kinase in Arabidopsis. Plant Cell. 24(5): 2225-36.

18. Johnson K, Dong OX, and Li X (2011). The evolutionarily conserved MOS4-associated complex. Central European Journal of Biology. 6(5): 776-784.

19. Li Y, Dong OX, Johnson K, and Zhang Y (2011). MOS1 epigenetically regulates the expression of plant Resistance gene SNC1.Plant Signal Behav. 6(3): 434-6.

20. Dong OX, Johnson K, Li X, and Zhang Y (2011). The mRNA export pathway in plants. Frontiers in Biology. 6(3): 246–250.

21. Germain H, Qu N, Cheng Y, Lee E, Huang Y, Dong OX, Gannon P, Huang S, Ding P, Li Y, Sack F, Zhang Y, and Li X (2010). MOS11: a new component in the mRNA export pathway.PLOS Genetics. 6(12): e1001250.

奖励与荣誉

南京市留学回国人员择优资助（A类）2022

澳门十大正规老牌网赌教师教学创新大赛团队优秀奖2022

江苏特聘教授2021